Grade 7 Integrated Science · Strand 1: Scientific Investigation · Laboratory Apparatus & Instruments
Operating the Light Microscope
A short Grade 7 Integrated Science lesson for the CBE curriculum, part of Scientific Investigation: Laboratory Apparatus & Instruments. Learn it on any phone with notes and slides, then check yourself with a quick quiz and earn XP. This lesson is free.
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1. A learner uses a ×10 eyepiece lens together with a ×40 high-power objective lens. What is the total magnification of the specimen? (Give the number only, without the × sign.)
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2. A learner looks at an onion wet mount and sees thick, perfectly circular dark rings covering the cells. What are these most likely to be?
- A. Trapped air bubbles under the coverslip
- B. Stained onion cell nuclei
- C. Grains of unused iodine
- D. Cracked pieces of the slide
3. Why should you use only the fine adjustment knob when viewing a specimen under the high-power (×40) objective lens?
- A. The coarse knob cannot turn once the nosepiece is rotated
- B. High-power lenses need more light from the mirror
- C. The long lens is very close to the slide, so coarse movement can smash the glass
- D. The specimen disappears if the stage moves downwards
4. Which of the following is the correct order for preparing a stained onion wet mount?
- A. Add iodine, peel tissue, place tissue in water, lower coverslip
- B. Place tissue in water, peel tissue, lower coverslip, add iodine
- C. Peel tissue, place tissue in water on the slide, add iodine, lower coverslip at 45 degrees
- D. Peel tissue, add iodine, lower coverslip, place tissue in water
5. What name is given to the chemical dye, such as iodine, that is added to a slide to colour cell parts so they can be seen clearly?
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